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PAF- and TNF-α-induced IκBα promoter-directed CAT gene expression in transiently transfected CHO-PAFR cells. CHO-PAFR cells were transfected with 5 μg of IκBα promoter-CAT reporter plasmids (specified at the top) or 5 μg of pSVLCAT plasmid, together with 1 μg of pCMVβ plasmid. CAT activity was determined in these cells after stimulation of PAF or TNF-α. The results shown here are representative of three CAT assays. N.D.: not detectable.
