FIG. 9.

Potential autocrine regulation of IL-8 synthesis. Left: schematic representation of a possible autocrine mechanism for IL-8-stimulated IL-8 production. Right: autoradiographs of an EMSA (top) showing IL-8 induced KB binding activity, and of a Northern blot (bottom) depicting the accumulation of cytoplasmic IL-8 message in the same cells. Freshly isolated blood mononuclear cells were treated with IL-8 at the indicated concentrations for 1 h before harvesting for the preparation of total RNA and nuclear extracts, as described in Materials and Methods.