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. 2017 May 4;88(0):1416. doi: 10.4102/jsava.v88i0.1416

TABLE 1.

Sequences of oligonucleotide primers used to target genetic determinants responsible for species confirmation, virulence and resistance in Staphylococcus aureus.

Target gene Primer sequence (5’ 3’) Product size (bp) References
nuc F: GCGATTGATGGTGATACGGTT 270 Brakstad et al. (1992)
R:AGCCAAGCCTTGACGAACTA AAGC
coa F: CGA GAC CAA GAT TCA ACA AG 730 Aslantas et al. (2007)
R: AAA GAA AAC CAC TCA CAT CA
spa F: CAA GCA CCA AAA GAG GAA 320 Frenay et al. (1996)
R: CAC CAG GTT TAA CGA CAT
sea F: GCA GGG AAC AGC TTT AGGC 521 Monday and Bohach (1999)
R: GTT CTG TAG AAG TAT GAAACA CG
see F: TAC CAA TTA ACT TGT GGA TAG AC 171 Monday and Bohach (1999)
R: CTC TTT GCA CCT TAC CGCA
mecA F: AAAATCGATGGTAAAGGTTGGC 532 Strommenger et al. (2003)
R: AGTTCTGCAGTACCGGATTTGC
BlaZ F: ACTTCAACACCTGCTGCTTTC 240 Martineau et al. (2000)
R: TAGGTTCAGATTGGCCCTTAG
tetK F: TTAGGTGAAGGGTTAGGTCC 718 Aarestrup et al. (2000)
R: GCAAACTCATTCCAGAAGCA