. 2018 Aug 19;596(18):4393–4412. doi: 10.1113/JP275996

Table 1.

Acute hypoxic ventilatory responses of mice after conditional inactivation of HIF‐2α in TH or GFAP expressing cells before and after chronic hypoxia exposure

Acute hypoxic stimulus	Chronic hypoxia duration	Hypoxic ventilatory response (mL min⁻¹ g⁻¹)			ANOVA
		Hif‐2α ^f/f	Hif‐2α ^f/f;THCreER	t test P value	P value
10% O₂	0 h	–0.05 ± 0.30	0.03 ± 0.34	0.858
	48 h	2.86 ± 0.33	1.70 ± 0.34	0.028	<0.0001
	7 days	4.24 ± 0.21	2.84 ± 0.45	0.013
ANOVA P value	0.0069				0.069
10% O₂/3% CO₂	0 h	3.32 ± 0.28	2.89 ± 0.30	0.314
	48 h	6.43 ± 0.56	4.58 ± 0.46	0.023	<0.0001
	7 days	9.13 ± 0.78	5.99 ± 0.42	0.003
ANOVA P value	<0.0001				0.012

Acute hypoxic stimulus	Chronic hypoxia duration	Hypoxic ventilatory response (mL min⁻¹ g⁻¹)			ANOVA
		Hif‐2α ^f/f	Hif‐2α ^f/f;GFAPCreER	t test P value	P value
10% O₂	0 h	–0.24 ± 0.40	0.82 ± 0.57	0.167
	48 h	1.30 ± 1.12	1.79 ± 0.85	0.804	0.0004
	7 days	3.70 ± 0.87	3.06 ± 0.42	0.530
ANOVA P value	0.631				0.405
10% O₂/3% CO₂	0 h	3.15 ± 0.36	3.06 ± 0.31	0.844
	48 h	6.66 ± 1.17	6.64 ± 0.49	0.987	<0.0001
	7 days	8.25 ± 1.02	7.51 ± 0.73	0.571
ANOVA P value	0.647				0.873

Hypoxic ventilatory responses of Hif‐2α^f/f;THCreER and Hif‐2α^f/f;GFAPCreER mice vs. Hif‐2α^f/f littermate controls. Measurements were taken after tamoxifen‐induced recombination and before (0 h) and after (48 h, 7 days) exposure to 10% oxygen. Values shown are mean ± SEM changes in minute ventilation in response to the indicated acute hypoxic stimulus; n = 5–12 littermate pairs for each genotypic comparison. Significance was tested using two‐way ANOVAs (right hand column P value = chronic hypoxia factor; bottom row P value = genotype factor; right column, bottom row P value = chronic hypoxia/genotype interaction factor), followed by t tests (with Holm–Sidak correction) for analysis of individual time points; P < 0.05 highlighted in bold.