Table 1. Sequence and characterization of primers used in this study.

Gene		Sequences	Size	TM	Cycles
ACTB	Forward	CCATCGAGCACGGCATCGTCACCA	376 bp	68°C	22 cycles
	Reverse	CTCGGTGAGGATCTTCATGAGGTAGT
KLOTHO (KL)	Forward	ACTCCCCCAGTCAGGTGGCGGTA	350 bp	68°C	37 cycles
	Reverse	TGGGCCCGGGAAACCATTGCTGTC
KL-U1 (U)	Forward	AGAGGATGTGTGGTAGGTAAAGAG	213 bp	62°C	34 cycles
	Reverse	ACAAACCAAAACTACCTCCACCCT
KL-M1 (M)	Forward	AGAGGACGCGCGGTAGGTAA	213 bp	59°C
	Reverse	ACGAACCGAAACTACCTCCGC
KL-U2 (U)	Forward	GGTTTGGGTGGTTGTTGTTTGTG	154 bp	62°C	37 cycles
	Reverse	TCTAATAAACAACACTACCCACAACC
KL-M2 (M)	Forward	GGCGGTCGTCGTTTGCGT	144 bp	62°C
	Reverse	ATAAACGACGCTACCCACGAC
KL-U3 (U)	Forward	GGGGGAATTTTTTTTAGTGTATGGT	188 bp	61°C	33 cycles
	Reverse	TACCAAACCCTAACAAAACACA
KL-M3 (M)	Forward	GGGAATTTTTTTTAGCGTACGGC	184 bp	63°C
	Reverse	CTACCGAACCCTAACGAAACG
KL-U4 (U)	Forward	GTTAGTGGAGTTTGTTGGGGAGT	186 bp	65°C	36 cycles
	Reverse	AACACCAACAACAACAACAACAACA
KL-M4 (M)	Forward	GTTAGCGGAGTTCGTCGGG	184 bp	63°C
	Reverse	CCAACAACAACGACAACGACG
KL-BSP	Forward	GGTAATAGTAAAAGGGAGAGTAAAATTT	465 bp	54°C	40 cycles
	Reverse	AAAAAACACCTATTTCTCCCAAC
KL-Pyro	Forward	TGGGTTTTYGAGTGGGAGAAAAGTGA	234 bp	58°C	45 cycles
	Reverse	CACCTATTTCTCCCAACTCC
	Sequencing	AGTGAGAGTAGGTGT