Figure 5.

Regulation of MPZ expression by the PI3K/Akt pathway. As shown in (A) by western blotting analysis, before stimulated with 150 ng/ml recombinant NT-4, Schwann cells were pretreated with 25 μM PD98059 for 1 h and 40 μM LY294002 for 2 h. PD98059 did not show inhabitation to the elevation of MPZ expression. In contrast, LY294002 markedly inhibited the increase in the expression of MPZ (n = 5, p = .001). (B) The Akt phosphorylation was examined by western blotting analysis. The level of Akt phosphorylation was significantly increased in the presence of NT-4 stimulation compared with the absence of NT-4. Each bar represents the mean ± SEM of five independent experiments. *p < .05; **p < .01, NS, not significant.