Figure 1.

Effects of MG132 or 3-MA treatment on ataxin-3 degradation. To assess the involvement of different proteolytic pathways on ataxin-3 degradation, BOSC cells expressing HA-tagged ataxin-3 (26Q or 73Q) and FLAG-tagged SUMO-1 were incubated with 5 µM MG132 (a proteasome inhibitor) (A) or 5 mM 3-MA (an autophagy inhibitor) (B). After 24 h, cells were collected, lysed and the cell lysate was subjected to immunoblot analysis using anti-HA antibody (ataxin-3) and anti-β-actin antibody. Arrows indicate the SUMO-modified form of ataxin-3.