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. 2018 Jun 28;22(4):227–233. doi: 10.1080/19768354.2018.1490348

Figure 3.

Figure 3.

The changes of p-mTOR and p-AMPKα1 expressions during food deprivation in the dorsal horn and the spinal cord. The mice were forced into food deprivation for 12, 24, or 48 h. Then, DRG and the lumbar region of the spinal cord were dissected. p-mTOR and p-AMPKα1 expressions in the spinal cord and the DRG were analyzed by Western blot. The number of animals in each group was 6. β-Actin (1:1000 dilution) was used as an internal loading control. Signals were quantified with the use of laser scanning densitometry and expressed as a percentage of the control. Values are mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001; compared to Control group)