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. 2017 May 18;21(3):152–159. doi: 10.1080/19768354.2017.1324522

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Confirmation of microarray analysis. (A) Confirmation of gene expression according to microarray data using RT-PCR. SVZ- or SCZ-enriched genes were selected randomly, and bar graphs show the ratio of mRNA expression difference between two aNSCs populations upon normalization with GAPDH (N = 4). (B) Gene candidates were selected and confirmed by Western blot (N = 2), and representative images were shown. (C) CRBP1 and (D) HMGA1 proteins were immunostained with Nestin, a marker for NSCs, in the SVZ and SCZ, respectively. Magnified image of the box region is shown as an inset. Hoechest33343 was used as a nuclear marker.