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. 2017 Jul 1;21(4):241–254. doi: 10.1080/19768354.2017.1342691

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Change in cell morphology and senescence-associated-β-galactosidase activity in untreated control (A, B and C) and 1 μM SMA-treated (D, E and F) A-549, MDA-MB-231 and U87-MG cancer cells A-549, MDA-MB-231 and U87-MG cancer cells up to 2 weeks (×100). Cell changes, such as enlarged and flattened size, and star-shape, were frequently observed by SMA treatment. And a higher activity of senescence-associated-β-galactosidase stained with blue color were observed in cells treated with SMA. Scale bars: 50 μm.