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. 2017 Jul 1;21(4):241–254. doi: 10.1080/19768354.2017.1342691

Figure 5.

Figure 5.

Morphological evaluation of cellular apoptosis and differentiation in 1 μM SMA-treated A-549 (A), U87-MG (B) and MDA-MB-231 (C and D) cancer cells. The fragmented nuclei (arrow) displayed in apoptotic cells were observed by Wright-Giemsa staining, compared with normal (double arrow) nuclei. Further, lipid droplets (dotted arrow)-like morphological alternations under light microscope (C) were observed by 1 μM SMA treatment and the droplets were stained with Oil red O solution (D). (E) Frequency of apoptotic cells with DNA fragmentation in untreated control (▪) and 1 μM SMA-treated (▪) A-549, MDA-MB-231 and U87-MG cancer cells. Asterisks (*) indicates significant (P < .05) difference between untreated control and SMA-treated cell lines, respectively.