Table 1. Primer sequences, PCR product size and annealing temperature used for RQ-TRAP and RT-PCR.
| Gene | Primer sequences (5′–3′) | Amplification size (bp) | Annealing temp (°C) |
|---|---|---|---|
| RQ-TRAP TS |
AATCCGTCGGAGCAGAGTT | 60 | |
| RQ-TRAP ACX |
GCGCGGCTTACCCTTACCCTTACCCTAACC | 60 | |
| GAPDH | GAAGGTGAAGGTCGGAGTC GAAGATGGTGATGGGATTTC |
228 | 57 |
| TERT | CGGAAGAGTGTCTGGAGCAA GGATGAAGCGGAGTCTGGA; | 198 | 60 |
| TERC | TCTAACCCTAACTGAGAAGGGCGTAG, GTTTGCTCTAGAATGAACGGTGGAAG |
126 | 60 |
| BAX | TCTGACGGCAACTTCAACTG AGTCCAATGTCCAGCCCATG |
127 | 60 |
| Caspase-3 | TGAGCCATGGTGAAGAAGGA TCGGCCTCCACTGGTATTTT |
220 | 55 |
| Caspase-9 | CTCTTGAGAGTTTGAGGGGAAA ACTCACGGCAGAAGTTCACA |
105 | 55 |
| p21 | TGGCAGTAGAGGCTATGGA AACAGTCCAGGCCAGTATG |
178 | 57 |
| HSP70 | ACGAATCCCTGCGGTAAAAG AAAGCAGCGATAAGATGGC |
127 | 60 |
| HSP90 | ACAAGCACATATGGCTGGAC TCTTTGCTGCCATGTAACCC |
94 | 58 |