Figure 4.

mRNA expression levels of SREBP target genes in human fibroblasts transfected with miR-96. Total RNA was prepared from cells transfected with either miR-NC or a miR-96 mimic. The expression levels of the target genes of SREBP-1 (FAS, SCD-1, and ELOVL6) and SREBP-2 (HMG-CoA synthase and HMG-CoA reductase) were measured. The expression levels in cells without transfection were regarded as 1.0 and levels in other groups determined relative to this group. Data were normalized to a cyclophilin endogenous control (n = 2). FAS, fatty acid synthase; SCD-1, stearoyl-CoA desaturase; ELOVL6, elongation of very long chain fatty acid-like family member 6.