Fig 2. Radiolabelled cholesterol accumulation in the bile and faeces.
The amount of cholesterol accumulation in the bile (A) was determined by measuring a known volume by liquid scintillation counting. Cholesterol accumulation in the faeces (B) as was determined by lipid extraction using the Bligh-Dyer method and then quantified by liquid scintillation counting. Both measures were then expressed as a % of the total amount of radiolabelled cholesterol injected. *p = 0.002; **p<0.0001.