Skip to main content
. 2018 Sep 14;13(9):e0203745. doi: 10.1371/journal.pone.0203745

Fig 4. Effect of PKM2 inhibition by si-RNA on HIF-1α.

Fig 4

PC3 and LNCaP cells were transfected and cultured for 24 h in normoxia 20% O2 or hypoxia 0.1% O2. (A) HRE reporter gene assay of transfected cells with si-PKM2 (n = 6, Mean ± SEM, *** P < 0.001, **** P < 0.0001, §§§§ P < 0.0001 vs si-con Hox). (B) VEGF mRNA expression detection and quantification by qRT-PCR after treatment of cells with si-PKM2 (n = 3, Mean ± SEM, ** P < 0.01 vs si-con HoX). (C) Western blot analysis of HIF-1α protein levels after si-PKM2 transfection and validation of the suppressive effects of si-PKM2 on PKM2 and PKM2++ (Tyr105) protein levels. (D) Densitometric analysis of HIF-1α western-blot normalized to β-actin after treatment of cells with si-PKM2.