Fig 5. PHD3 expression and colony survival in prostate cancer cell lines.

PC3 and LNCaP cells were transfected and cultured for 24 h in normoxia 20% O2 or hypoxia 0.1% O2. (A) PHD3 mRNA expression detection and quantification by qRT-PCR (n = 3, Mean ± SEM, *** P < 0.001 vs NOX). (B) Colony survival assay in transfected cells with si-PKM2, (n = 3, Mean ± SEM, ** P < 0.01, **** P < 0.0001, ^§§§§ P < 0.0001 vs si-con Hox).