Table 1. Bacterial richness and diversity in Triatoma sordida’s gut microbiota through bug development.
Bacterial Operational Taxonomic Units (OTUs) were defined based on a 97% 16S rRNA sequence identity cutoff.
| Sample* | No. observed OTUs | Chao1 richness estimator (mean±SE OTUs) | Shannon’s diversity index (mean±SE bits) |
|---|---|---|---|
| 1I | 23 | 25.0 ± 2.30 | 1.44 ± 1.05 |
| 2I | 41 | 42.2 ± 1.20 | 2.77 ± 1.12 |
| 3I | 36 | 37.0 ± 1.25 | 2.94 ± 1.10 |
| 4I | 51 | 49.0 ± 1.50 | 3.18 ± 2.10 |
| 5I | 50 | 50.0 ± 2.10 | 3.35 ± 1.25 |
| FI | 51 | 48.0 ± 3.00 | 3.26 ± 1.20 |
| MI | 51 | 50.0 ± 2.10 | 3.37 ± 1.20 |
*Samples: 1I–3I, whole intestine of 1st to 3rd stage nymphs; 4I to MI, pooled results from separately processes samples from the anterior midgut, posterior midgut, and hindgut of 4th and 5th stage nymphs (4I and 5I, respectively) and adult female and male bugs (FI and MI, respectively)