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. 2018 Sep 4;14(9):e1007626. doi: 10.1371/journal.pgen.1007626

Fig 4. The SUMO protease ULP-1 is required for RC disassembly.

Fig 4

(A) Spindles stained for DNA (blue), tubulin (green), SUMO (not shown in merge) and AIR-2 (red) following control, 5 day ulp-1(RNAi), or 24–48 hour ulp-1(RNAi). In 75/75 control pre-anaphase spindles, SUMO and AIR-2 mark 6 RCs (row 1). In 40/44 anaphase spindles, RC disassembly was normal (row 2). Following 5 day ulp-1(RNAi), SUMO was not observed on the RCs in 65/71 pre-anaphase spindles (row 3). Of the 10 anaphase spindles that contained SUMO, three of the spindles had RC disassembly defects (row 4). In addition, we observed three spindles that had a failed meiosis phenotype (row 5). Following 24–48 hour ulp-1(RNAi), SUMO was observed on all six RCs in 112/142 pre-anaphase spindles (row 6). Of the 103 anaphase spindles that contained SUMO, 27 of the spindles had RC disassembly defects (row 7). We also observed six spindles with a failed meiosis phenotype (bottom). Bar = 2.5μm. (B) Stacked bar graph illustrating the percent of spindles with 0, 1, 2, 3, 4, 5, or 6 SUMO rings in control spindles versus 5 days, 72, 48, and 24 hours of feeding RNAi. This analysis illustrates the sharp transition in the number of SUMOylated RCs between 48 and 72 hour depletion.