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. 2018 Sep 4;14(9):e1007626. doi: 10.1371/journal.pgen.1007626

Fig 5. BUB-1 targets ULP-1 to the RC to promote disassembly.

Fig 5

(A) ULP-1 (red) localization from diakinesis through late anaphase in wild-type oocytes (rows 1–6), compared to AIR-2 (green, column 4), DNA (blue), and tubulin (green, columns 1 and 5). ULP-1 begins to concentrate on the RCs during spindle formation and then leaves the RCs in mid anaphase. When RC disassembly is delayed in the him-8(me4) mutant, ULP-1 and AIR-2 remain associated with the RCs (row 7). (B) Anaphase spindle showing an AIR-2 (red) relocalization defect (quantification on right) after partial bub-1(RNAi). Quantification of this AIR-2 relocalization defect on right includes both 48 and 72 hour bub-1(RNAi). (C) Collapsed spindle following partial bub-1(RNAi) stained for ULP-1 (red), compared to DNA (blue), tubulin (green, columns 1 and 5), and AIR-2 (green, column 4). ULP-1 localizes to chromosomes, but is not RC-associated. (D) Partial bub-1(RNAi) spindles in late anaphase I (row 1) and prometaphase II (row 2) spindles, stained for SUMO (red), compared to DNA (blue), tubulin (green, columns 1 and 5), and AIR-2 (green, column 4). Defects in RC disassembly lead to persisting SUMO and AIR-2 structures. Images shown in B, C, and D include examples of 48 hour and 72 hour RNAi. Bar = 2.5μm.