Fig. 1.

Cooperative effect of transforming growth factor-beta-1 and type I collagen substratum on alteration in cell phenotype. Human kidney-2 cells were grown on a plastic surface (A–C) or type I collagen substratum (D–F) to confluence followed by serum deprivation for 2 days. Cells were then incubated in the absence (A and D) or presence of transforming growth factor-beta-1 (10 ng/mL) (B, C, E and F) for 3 days. Subsequently, stability of cell phenotype was determined by removal of transforming growth factor-beta-1 and addition of medium containing 10% fluorescence correlation spectroscopy for further 3 days (C and F). Cell morphology was monitored and photographed by phase contrast microscopy, ×100.