Fig. 3.

Effect of transforming growth factor-beta-1 and contact with type I collagen on an epithelial marker, E-cadherin expression. Cells were grown on a plastic surface (A and B) or type I collagen substratum (C and D) in the absence (A and C) or presence (B and D) of transforming growth factor-beta-1 (10 ng/mL) under serum free condition for 3 days. Subsequently, cells were fixed with 3.5% paraformaldehyde and E-cadherin expression was assessed by immunocytochemistry and confocal microscopy as described in materials and methods, ×400.