Fig. 9.

Stimulation of matrix metalloproteinases-9 activity following addition of transforming growth factor-beta-1. Confluent monolayers of cells were stimulated with recombinant transforming growth factor-beta (10 ng/mL) for up to 4 days (A) or increasing doses (0, 10, 50 ng/mL) for 4 days (B). Supernatant samples were collected and gelatinase activity assessed by substrate zymography as described in Materials and methods.