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. 2018 Aug 9;37(18):e99777. doi: 10.15252/embj.201899777

Figure EV3. SILAC.

Figure EV3

  1. Labeling culture conditions and numbers of identified proteins.
  2. APB Northern blot using tRNAHis probe and C38 methylation of tRNAAsp in SILAC labeled cells.
  3. SILAC analysis of deregulated proteins in the absence of Q. The top 10% of down‐regulated proteins are indicated in red, the top 10% of up‐regulated proteins are indicated in blue, HSPA5/BiP is indicated in orange.
  4. Increase or decrease in a codon frequency (‰) in up‐ or down‐regulated proteins relative to the average frequency of that codon in unchanged proteins is displayed as heatmap according to the color scale.
Data information: DS (dialyzed medium), q (queuine), Q (queuosine), and G (guanine).Source data are available online for this figure.