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. 2018 Aug 14;37(18):e95329. doi: 10.15252/embj.201695329

Figure 5. chr7 piRNA cluster directs de novo DNA methylation of the Rasgrf1 DMR .

Figure 5

  1. Structure of the chr7 piRNA cluster. Top gray bars represent a putative piRNA precursor predicted by H3K4me3 ChIP‐seq, RNA‐seq, and small RNA‐seq. Only uniquely mapped reads were used for displaying the sequencing result. For RNA‐seq and small RNA‐seq, only the results of minus strand are shown, because plus strand does not express appreciable level of RNA. A promoter region deleted is shown above the putative piRNA precursor. RPM, read per million.
  2. Low expression level of piRNAs from chr7 cluster in chr7 piRNA cluster Δpromoter/Δpromoter testes. Unchanged expression of piRNAs from chr10 cluster (bottom) shows chr7 cluster is specifically affected. Only uniquely mapped piRNAs are shown.
  3. DNA methylation status of the Rasgrf1 differentially methylated region (DMR) in spermatogonia from chr7 piRNA cluster Δpromoter/Δpromoter. See Fig 4A and B for information about the region examined.