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. 2018 Aug 14;37(18):e95329. doi: 10.15252/embj.201695329

Figure EV5. Ping‐pong cycle within each hypomethylated region.

Figure EV5

  1. Bidirectional piRNA expression from HMR_−L1s. Direction index is defined as indicated. When half (all) of piRNAs are derived from the same strand, direction index is 0.5 (1). Only unique piRNA reads were used for this analysis. HMR_−L1s and mRNAs that generate appreciable level of unique piRNAs (≥ 10 unique piRNAs) were selected for this analysis. We divided them into two groups according to the number of generating unique piRNAs (10–99 and ≥ 100, top and bottom panels) in order to rule out the possibility that the difference is arisen from the number of piRNAs analyzed. The numbers of HMR_−L1s and mRNAs analyzed are shown in parentheses.
  2. Bidirectional RNA expression from HMR_−L1s. HMR_−L1s, transcription start sites (TSSs), and 2nd exons were divided into two groups according to their RNA expression levels (0.5–5 and 5–50), and they were analyzed separately (top and bottom panels). We considered only unique RNA‐seq reads that were mapped within 2‐kb regions spanning the centers of HMR_−L1s, TSSs, and start sites of 2nd exons (positions −1,000 to 999, when they are considered to be position 0).
  3. Two representative HMR_−L1s generating sense and antisense piRNAs.
  4. High frequency of 10‐nt overlap between 5′ ends of uniquely mapped sense and antisense piRNAs from HMR_−L1s. Heatmap shows relative frequencies among 1‐ to 25‐nt overlaps in each HMR_−L1.