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. 2018 Aug 14;37(18):e98732. doi: 10.15252/embj.201798732

Figure 2. Irc5 is required for S phase progression in the presence of MMS‐induced DNA damage.

Figure 2

  1. The irc5‐Δ1 mutant exhibits S phase delay in the presence of MMS. DNA content of wild‐type (W303‐1a) and irc5‐Δ1 (IL012) cells was measured by FACS and plotted as histograms.
  2. Completion of DNA replication is delayed in the irc5‐Δ1 mutant after MMS treatment. Intact chromosomes were isolated from wild‐type (W303‐1a) and irc5‐Δ1 (IL012) cells and processed for PFGE. M, 0.03% MMS.
  3. Quantification of data presented in (B). Error bars represent mean value ± standard deviations of mean for the intensity values of three distinct chromosome bands representing chromosome XII or III (n = 3). Values for G1 were set to 100.
  4. Samples used for PFGE in (B) were processed for FACS analysis.

Source data are available online for this figure.