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. 2018 Aug 28;37(18):e100158. doi: 10.15252/embj.2018100158

Figure 1. Identification of novel REV7 interactors relevant for the NHEJ pathway.

Figure 1

  1. Venn Diagram representing the distribution of proteins identified by both the BioID and the standard AP/MS of REV7, with or without DNA damage (NCS).
  2. Selected BioID REV7 results, shown as dot plots. The spectral counts for each indicated prey protein are shown as AvgSpec. Proteins were selected based on and iProphet probability of > 0.95, BFDR of < 0.05, and ≥ 10 peptide count. The circle size represents the relative abundance of preys over baits.
  3. U2OS EJ5‐GFP cells were transfected with the indicated siRNAs. At 24 h post‐transfection, cells were transfected with the I‐SceI expression plasmid, and the GFP+ population was analyzed 48‐h post‐plasmid transfection. The percentage of GFP+ cells was determined for each individual condition and subsequently normalized to the non‐targeting condition (siCTRL). Data are presented as the mean ± SD (n = 3). Significance was determined by one‐way ANOVA followed by a Dunnett's test. *P < 0.05, **P < 0.005, ***P < 0.0005.