Figure 8. TRIM16 reduces cytotoxicity mediated by oxidative and proteotoxic stresses.

• A
  MTT assays performed at different time points with HeLa and TRIM16^KO cells, untreated or treated with H₂O₂ (400 μM, 2 h). Data, mean ± SD, n = 3, *P < 0.05 (Student's unpaired t‐test).
• B
  Images of HeLa and TRIM16^KO cells, untreated or treated with As₂O₃ (2.5 μM, 4 h). Scale bar: 400 μm.
• C–F
  Flow cytometry analysis of HeLa and TRIM16^KO cells stained with Annexin‐V/propidium iodide (double‐staining), untreated or treated with (C) As₂O₃ (2.5 μM, 4 h), (D) H₂O₂ (400 μM, 2 h), (E) puromycin (5 μg/ml, 6 h), and (F) MG132 (20 μM, 8 h).
• G
  Immunoblot blot analysis of HeLa and TRIM16^KO lysates of cells treated with 5 μM of As₂O₃ for different durations as indicated and probed with antibodies as shown. L.E, low exposure; H.E, high exposure. Arrowheads indicate the cleaved form of PARP‐1 and caspase‐3.

Source data are available online for this figure.