Figure 9. TRIM16 is protective against oxidative stress‐induced cytotoxicity in vivo .

• A, B
  Clonogenic assays were performed with HeLa and TRIM16^KO cells, untreated or treated with As₂O₃ or MG132.
• C
  Tumor volumes of HeLa and TRIM16^KO tumors at indicated time points. As₂O₃ is injected when tumor volume of all groups was > 100 mm³ (day 0 in the graph). Tumor volumes were measured on an interval as indicated. Mean ± SE, n = 6 (each group), *P < 0.05, **P < 0.005, ***P < 0.0005 (ANOVA).
• D
  Graph shows the % tumor regression (tumor volume on day 1 of As₂O₃ treatment/tumor volume on day of sacrifice × 100). Mean ± SE, n = 6 (each group), **P < 0.005 (Student's unpaired t‐test).
• E
  Representative images of HeLa and TRIM16^KO tumors formed in the nude mice in the absence and presence of As₂O₃.
• F
  Representative pictures of dissected tumors.
• G
  Immunohistochemistry analysis performed with KI‐67 cell proliferation marker and hematoxylin nuclear stain. The graph represents the % of cells with KI‐67 staining. > 400 cells were counted for this analysis from three different sections from different animals, mean ± SD, *P < 0.05 (Student's unpaired t‐test). Scale bar: 50 μm.
• H
  WB analysis of tumor tissue lysates from two different mice with antibodies as indicated. L.E, low exposure; H.E, high exposure.
• I
  Graphical representation of work presented in this study.

Source data are available online for this figure.