. Author manuscript; available in PMC: 2019 Oct 1.

Published in final edited form as: Dev Biol. 2018 Jun 6;442(1):87–100. doi: 10.1016/j.ydbio.2018.06.005

Table 1:

List of primers used within this study

Gene Name	Forward: 5’ → 3’	Reverse: 5’ → 3’
Nkx2.1	`GGTAGTTCAAATGGGTTCCCAAGC`	`AGTTCAGGGCCATGGAAGCATAG`
Pome	`TGGCTCAATGTCCTTCCTGG`	`CACATAAGCTGCATCGTTAAG`
Kissl	`TGCTGCTTCTCCTCTGT`	`ACCGCGATTCCTTTTCC`
Gfap	`TGAATCGCTGGAGGAGGA`	`TGTGAGGTCTGGCTTGGC`
Lhx2	`GCCAAGGACTTGAAGCAGCT`	`GGTTGCGCCTGAACTTGGCC`
Sox2	`GGAGAAAGAAGAGGAGAGAG`	`CTGGCGGAGAATAGTTGG`
Sox9	`AGCCGACTCCCCACATTCCTCC`	`GAGATTGCCCAGAGTGCTCGCCC`
Notch 1	`TTCCATGATTGCCTCCTGCAGTGG`	`CAGGATCAGTGGAGTTGTGCCATCATGCA`
Notch2	`TGCCAATACTCCACCTCTC`	`TCCACTGACACTGCTTCC`
Notch3	`GAAGACCCTTGCCACTCAG`	`CCACGGAGACAACGACAG`
Notch4	`GAGGACCTGGTTGAAGAATTGATC`	`TGCAGTTTTTTCCCTTTTATCCC`
Dili	`TAACCTCGTTCGAGACCTCAAGGG`	`TGGCACTTGGTGTCACGTTTGC`
DII3	`TCTCCCTCGTCATTGAAACCTGGA`	`GTAGGAGAAGTGCAACTCCATGT`
Jag1	`TGTAGCCAGAAAGTGACTGGTTG`	`TGTAGCCAGAAAGTGACTGGTTG`
Jag2	`TGGACACGAAAGCGCAGGAAAG`	`ACTGGTTGTTGGCGCTCTCATC`
Hey1	`TCTTGCAGATGACTGTGG`	`ATGATGCTCAGATAACGG`
Hes1	`AAATGACTGTGAAGCACC`	`TCATGCACTCGCTGAAGC`
Hes5	`CGCATCAACAGCAGCATAGAG`	`TGGAAGTGGTAAAGCAGCTTC`
Mash1	`TGGACTTTGGAAGCAGGATGG`	`TGACGTCGTTGGCGAGAAACA`
Rpl7	`CGCACTGAGATTCGGATG`	`TTAATTGAAGCCTTGTTGAGC`
Ppia	`CAAATGCTGGACCAAACACAAACG`	`GTTCATGCCTTCTTTCACCTTCCC`
mKi67	`CCAGGGATCTCAGCGCAATTACAG`	`GGATAGGACAGAGGGCCACATTC`
Olig2	`CTAATTCACATTCGGAAGGTTG`	`GGACGATGGGCGACTAGAC`
Nes	`AGCAGGGTCTACAGAGTCAG`	`GTCCTGTATGTAGCCACTTCC`
Lhx2	`GCCAAGGACTTGAAGCAGCT`	`GGTTGCGCCTGAACTTGGCC`
Bax	`TGAAGACAGGGGCCTTTTTG`	`AATTCGCCGGAGACACTCG`
Bcl2	`ATGCCTTTGTGGAACTATATGGC`	`GGTATGCACCCAGAGTGATGC`