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. Author manuscript; available in PMC: 2018 Sep 14.
Published in final edited form as: ACS Infect Dis. 2018 Jul 30;4(9):1377–1384. doi: 10.1021/acsinfecdis.8b00104

Figure 1.

Figure 1

Overview of the direct-qPCR assay for N. gonorrhoeae identification and AST. In this streamlined workflow, N. gonorrhoeae cells are inoculated in GW medium. The inoculated medium is aliquoted for each test condition and treated with different antimicrobials as needed. A direct-qPCR assay is performed using a real-time PCR instrument, followed by a built-in melting curve analysis step to verify amplicon specificity. Phenotypic responses to tested antimicrobial conditions (as a function of genomic DNA replication) are determined by the difference in Cq between test reaction and antimicrobial-free control reaction (ΔCq).