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. Author manuscript; available in PMC: 2018 Sep 14.
Published in final edited form as: ACS Infect Dis. 2018 Jul 30;4(9):1377–1384. doi: 10.1021/acsinfecdis.8b00104

Figure 2.

Figure 2

Use of direct-qPCR assay for quantitation of N. gonorrhoeae in GW medium. (A) Direct-qPCR quantifies cells ranging from 107 CFU/mL to 102 CFU/mL. Cq values were obtained using regression fit determined by the BioRad CFX Manager v3.1. Amplification curves are highly consistent across technical replicates, although some stochastic effects were observed when only a few target molecules (102 CFU/mL) are present in the sample. (B) Linear fit of the quantitative Cq values using Origin Pro 2017 with instrumental weighting. PCR efficiency of approximately 100% was obtained from the slope value of the linear fit.