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. Author manuscript; available in PMC: 2018 Sep 14.
Published in final edited form as: ACS Infect Dis. 2018 Jul 30;4(9):1377–1384. doi: 10.1021/acsinfecdis.8b00104

Figure 4.

Figure 4

Growth measurement of N. gonorrhoeae in complex sample matrices. (A) N. gonorrhoeae was spiked with one of nine urinary tract pathogens and commensals at a concentration of 104 CFU/mL and cocultured. All samples were cultivated at 37 °C for 6 h and a 10 μL aliquot of each sample was collected at 1 h interval for direct-qPCR analysis. No significant interference of N. gonorrhoeae growth is observed. (B) Five individual N. gonorrhoeae negative vaginal swabs were eluted in 500 μL of GW. Then each eluent was diluted 10-fold with GW medium and final concentration of 104 CFU/mL of N. gonorrhoeae cells was inoculated. The samples were cultivated at 37 °C for 6 h, and a 10 μL aliquot of each sample was collected at 1 h interval for direct-qPCR analysis. The growth of N. gonorrhoeae is not significantly affected by the contents eluted from the vaginal swabs.