Table 1.

EC₅₀ values of the alanine scan of 21-CSP and 18-CSP against S. mutans SAB249^a

Name	EC50 (nM)b	95% CIc	Fold changed	Name	EC50 (nM)b	95% CIc	Fold changed
21-CSP	240	200 – 290		18-CSP	6.2	3.7 – 10.0
21-CSP-S1A	90	43 – 190	0.38	18-CSP-S1A	2.6	1.1 – 6.5	0.42
21-CSP-G2A	140	60 – 330	0.58	18-CSP-G2A	9.7	4.2 – 22	1.6
21-CSP-S3A	150	100 – 230	0.63	18-CSP-S3A	13	7.1 – 24	2.1
21-CSP-L4A	6500	3300 – 13000	27	18-CSP-L4A	57	25 – 130	9.2
21-CSP-S5A	330	140 – 760	1.4	18-CSP-S5A	11	6.8 – 17	1.8
21-CSP-T6A	280	240 – 320	1.2	18-CSP-T6A	6.0	3.3 – 11	0.97
21-CSP-F7A	--e	--	--	18-CSP-F7A	220	130 – 370	35
21-CSP-F8A	--e	--	--	18-CSP-F8A	>1,000	--	--
21-CSP-R9A	1100	760 – 1500	4.6	18-CSP-R9A	27	14 – 51	4.4
21-CSP-L10A	370	200 – 680	1.5	18-CSP-L10A	6.5	3.5 – 12	1.1
21-CSP-F11A	--e	--	--	18-CSP-F11A	410	300 – 560	66
21-CSP-N12A	--e	--	--	18-CSP-N12A	33	18 – 60	5.3
21-CSP-R13A	480	390 –580	2.0	18-CSP-R13A	2.7	1.3 – 5.6	0.44
21-CSP-S14A	160	69 – 370	0.67	18-CSP-S14A	4.5	2.8 – 7.3	0.73
21-CSP-F15A	--e	--	--	18-CSP-F15A	--e	--	--
21-CSP-T16A	1400	980 – 2100	5.8	18-CSP-T16A	64	49 – 83	10
21-CSP-Q17A	94	61 – 140	0.39	18-CSP-Q17A	19	11 – 31	3.1
21-CSP-L19A	190	85 – 420	0.79
21-CSP-G20A	190	79 – 430	0.79
21-CSP-K21A	290	220 – 390	1.2

^aSee experimental section for details on reporter strain and methods. See supporting information for primary screening assay results and plots of agonism dose response curves. All assays were performed in triplicate.

^bEC₅₀ values determined by testing peptides over a range of concentrations.

^c95% confidence interval.

^dRatio where each analog’s EC₅₀ is divided by native CSP EC₅₀; a value <1 indicates a better activator than the parent CSP.

^eEC₅₀ not determined due to the analog’s low induction in primary agonism screening assay.

^fGreen color indicates modifications that improve processing of 21-CSP by SepM, purple color indicates modifications that decrease processing of 21-CSP by SepM.

^gRed color indicates modifications critical for 18-CSP:ComD binding.