Table 2.

EC₅₀ values of the D-amino acid scan of 21-CSP and 18-CSP against S. mutans SAB249^a

Name	EC50 (nM)b	95% CIc	Fold changed	Name	EC50 (nM)b	95% CIc	Fold changed
21-CSP	240	200 – 290		18-CSP	6.2	3.7 – 10.0
21-CSP-s1	140	100 – 190	0.58	18-CSP-s1	6.4	2.6 – 16	1.0
21-CSP-s3	160	71 – 370	0.67	18-CSP-s3	9.4	4.2 – 21	1.5
21-CSP-l4	390	160 – 920	1.6	18-CSP-l4	4.5	2.3 – 8.7	0.72
21-CSP-s5	640	410 – 1000	2.7	18-CSP-s5	17	6.4 – 45	2.7
21-CSP-t6	1600	1200 – 2100	6.7	18-CSP-t6	300	180 – 510	48
21-CSP-f7	--e	--	--	18-CSP-f7	>1,000	--	--
21-CSP-f8	840	440 – 1600	3.5	18-CSP-f8	42	33 – 52	6.8
21-CSP-r9	1500	860 – 2600	6.3	18-CSP-r9	76	32 – 180	12
21-CSP-l10	760	270 – 2200	3.2	18-CSP-l10	670	420 – 1100	110
21-CSP-f11	--e	--	--	18-CSP-f11	--e	--	--
21-CSP-n12	--e	--	--	18-CSP-n12	--e	--	--
21-CSP-r13	2100	1600 – 2700	8.8	18-CSP-r13	480	380 – 610	77
21-CSP-s14	--e	--	--	18-CSP-s14	>1,000	--	--
21-CSP-f15	--e	--	--	18-CSP-f15	--e	--	--
21-CSP-t16	--e	--	--	18-CSP-t16	>1,000	--	--
21-CSP-q17	--e	--	--	18-CSP-q17	130	90 – 200	21
21-CSP-a18	--e	--	--	18-CSP-a18	>1,000	--	--
21-CSP-l19	--e	--	--
21-CSP-k21	330	180 – 610	1.4

^aSee experimental section for details on the reporter strain and methods. See supporting information for primary screening assay results and plots of agonism dose response curves. All assays performed in triplicate.

^bEC₅₀ values determined by testing peptides over a range of concentrations.

^c95% confidence interval.

^dRatio where each analog’s EC₅₀ is divided by native CSP EC₅₀; a value <1 indicates a better activator than the parent CSP.

^eEC₅₀ not determined due to the analog’s low induction in primary agonism screening assay.

^fGreen color indicates modifications that improve processing of 21-CSP by SepM, purple color indicates modifications that decrease processing of 21-CSP by SepM.

^gRed color indicates modifications critical for 18-CSP:ComD binding.