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. Author manuscript; available in PMC: 2019 Sep 14.
Published in final edited form as: ACS Infect Dis. 2018 Jul 23;4(9):1385–1394. doi: 10.1021/acsinfecdis.8b00115

Table 3.

EC50 Values of the 2nd generation Analogs of 21-CSP and 18-CSP against the S. mutans SAB249a

Name EC50 (nM)b 95% CIc Fold changed Name EC50 (nM)b 95% CIc Fold changed
21-CSP 240 200 – 290 18-CSP 6.2 3.7 – 10.0
21-CSP-des-S1 23 19 – 28 0.10 18-CSP-des-S1 4.8 2.3 – 10.0 0.77
21-CSP-des-S1G2 120 49 – 310 0.50 18-CSP-des-S1G2 7.9 4.1 – 15 1.3
21-CSP-des-S1G2S3 530 340 – 810 2.2 18-CSP-des-S1G2S3 85 46 – 160 14
21-CSP-des-S1G2S3L4 3600 1500 – 8900 15 18-CSP-des-S1G2S3L4 570 270 – 1200 92
21-CSP-des-S1G2S3L4S5 3300 1600 – 6900 14 18-CSP-des-S1G2S3L4S5 950 460 – 2000 150
21-CSP-des-K21 98 52 – 180 0.41 18-CSP-des-A18 --e -- --
21-CSP-des-G20K21 3600 2100 – 6100 15 18-CSP-des-Q17A18 --e -- --
21-CSP-S3T 94 76 – 120 0.39 18-CSP-S3T 2.1 1.0 – 4.2 0.34
18-CSP-s1s3l4 17 11 – 26 2.7
18-CSP-s1s3l4L10AR13AS14A 3.4 2.9 – 4.00 0.55
18-CSP-S1AL10AR13AS14A 1.7 0.90 – 3.3 0.27
a

See experimental section for details on the reporter strain and methods. See supporting information for primary screening assay results and plots of agonism dose response curves. All assays performed in triplicate.

b

EC50 values determined by testing peptides over a range of concentrations.

c

95% confidence interval.

d

Ratio where each analog’s EC50 is divided by native CSP EC50; a value <1 indicates a better activator than the parent CSP.

e

EC50 not determined due to the analog’s low induction in primary agonism screening assay.