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. Author manuscript; available in PMC: 2018 Nov 10.
Published in final edited form as: Oncogene. 2018 May 10;37(32):4372–4384. doi: 10.1038/s41388-018-0257-5

Figure 6. Assessing the Na+/K+-ATPase as the potential target of Proscillaridin A.

Figure 6

(A–D) BxPC-3-KRASG12V and BxPC-3-KRASWT were transfected with siRNA targeting ATP1A1 or control siRNA and knockdown of the ATP1A1 subunit was confirmed by western blotting in cells grown in (A) 3D format or (B) 2D format. Viability of BxPC-3-KRASG12V and BxPC-3-KRASWT cells transfected with siRNA targeting ATP1A1 or control siRNA grown in grown in (C) 3D format or (D) 2D format was determined at 48 hours post-transfection using CTG3D or CTG, respectively. Statistical significance was determined by unpaired t-test. NS = Non significant, ** = p < 0.0001. The data shown represent the mean of 3 independent experiments with triplicate data points in each. Error bars = S.D. Levels of (E) pAKT, AKT and (F) pERK1/2, ERK1/2 were determined in BxPC-3-KRASG12V and BxPC-3-KRASWT cells grown in 3D format. Vinculin was used as a loading control.