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. 2017 Mar 21;40(1):49–54. doi: 10.1016/j.bj.2017.01.003

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© 2017 Chang Gung University. Publishing services by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Flow cytometry depicting B-regulatory cells (B-regs) characterized by CD19⁺ CD38^hi CD24^hi and IL10⁺. Representative dot plots of B-regs (A) in peripheral blood, (3.75%) and (B) after in-vitro generation, (27.49%). These plots show that there is rise in CD 19⁺ CD38^hi CD24^hi and IL10⁺ events after generation to 27.49% from baseline levels of 3.75% in peripheral blood depicting effective in vitro generation of B regs.