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. Author manuscript; available in PMC: 2019 Apr 17.
Published in final edited form as: Anal Chem. 2018 Mar 26;90(8):4935–4939. doi: 10.1021/acs.analchem.7b05246

Figure 2. Protein quantification using SDS-PAGE gel analysis and maXis II Q-TOF MS analysis for β-casein.

Figure 2.

(a) SDS-PAGE analysis of five different β-casein:5pβ-casein ratios from 1:5, 1:3, 1:1, 3:1 to 5:1 (top to bottom); (b) corresponding Q-TOF MS spectra (deconvoluted). In each deconvoluted spectrum, the relative abundance is normalized to the highest abundance species; (c) Correlation analysis between gas-phase ratios (derived from Q-TOF MS data) and solution-phase ratios (derived from SDS-gel data) of β-casein:5pβ-casein suggests a linear correspondence between these two ratios.