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. 2018 Sep 14;8:13836. doi: 10.1038/s41598-018-32095-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence microscopy analysis of brain and liver slices of NBD-gH625 treatment. Experimental classes are expressed in μg per 100 gr of body weight. Scale bar 50 μm. (a) Integrated density of brain slices from rats injected with different concentration of NBD-gH625. **P < 0.01, ***P < 0.001. (b) Integrated density of liver slices from rats injected with different concentrations of NBD-gH625. **P < 0.01, ***P < 0.001. (c) Calibration curve representing the linear relationship between the known amounts of NBD-gH625 and the relative the integrated density obtained under the setup of the microscopy analysis system used. (d) Percentage of NBD-gH625 delivered in brain and liver after in vivo administration of NBD-gH625.

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