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. 2018 Sep 14;8:13818. doi: 10.1038/s41598-018-32313-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative images of ion-treated zebrafish embryos used to determine heart rate at (a) 72 and (b) 96 hpf. (c) Mean heart rate of zebrafish embryos following 72- and 96-hpf treatments with indicated concentrations of different ions (Mg²⁺, Zn²⁺ and Ca²⁺). (d) Representative images of ion-treated zebrafish embryos used determine liver size at 96 h. (e) Mean liver size of zebrafish embryos after treatment with Mg (64 mM), Zn (500 μM), and Ca (128 mM) ions. * indicates significant difference compared to controls (p < 0.05).