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. 2018 Sep 14;9:3745. doi: 10.1038/s41467-018-06276-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — GMC ablation disrupts division axis maintenance. a Two successive divisions of NBs expressing worniu-GAL4-driven PH::GFP, the second division following the control ablation (upper panels) of a NB-neighbouring cell (yellow asterisk) away from the last-born GMC (blue asterisk) or following GMC ablation (lower panels) are shown. The ablation takes place between the two frames separated by a lightning symbol. Arrows: division axis. b Schematic of the ablations and angle measurements. c Deviation of the division axis following no ablation (Average angle: 18 ± 14°, n = 35), control ablation (23 ± 16°, n = 27), last-born GMC ablation (36 ± 20°, n = 35; also shown in Figs. 3c and 5c) or older GMC ablation (27 ± 16°, n = 26). Data from ten independent experiments. For examples of control or last-born GMC ablations resulting in a high misalignment of the second division axis, see Supplementary Movies 6 and 7. d Three successive divisions of NBs expressing worniu-GAL4-driven PH::GFP. The ablation takes place between the two frames separated by a lightning symbol. Blue asterisk: GMC prior to ablation, green asterisk: new last-born GMC. Arrows: division axis. e Schematic of the angles α measured from the movies shown in the previous panel. f Deviation α of the second (59 ± 14°, n = 9) and third (25 ± 11, n = 9) divisions described in the two previous panels. Data from five independent experiments. Scale bar in all panels: 5 µm