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. 2018 Sep 14;8:13802. doi: 10.1038/s41598-018-30800-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AAV8 plasmid bearing the GNMT gene and GNMT protein expression in the liver tissues. (a) Representative diagram of AAV8 injection in Gnmt^−/− (KO) mice. (b) The schematic construct of pAAV8-GNMT. (c) GNMT expression of 293T cells transfected with pAAV8-GNMT was measured by western blotting. The original picture of western blotting was provided in Fig. S1. (d) Staining for GNMT was performed on liver sections of WT, KO, KO-AAV8-eGFP and KO-AAV8-GNMT mice by immunohistochemical assessment.