Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 14;8:13804. doi: 10.1038/s41598-018-31772-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

hiPSC-CM morphology and sarcomeric organization. The results for the WT hiPSC-CMs are indicated in blue, those for the R219H hiPSC-CMs are in orange. (a) Immunocytochemistry of the WT and Na_v1.5/R219H hiPSC-CMs at D20 (top), 30 (middle) and 60 (bottom) of differentiation. The first column of each panel represents myosin light chain 2 v staining (mlc2v, green) and the second column represents cardiac troponin T staining (cTnT, red). The third column shows the merger of columns one and two with the DAPI nuclear staining. For the WT hiPSC-CMs, the nucleus cannot be clearly seen due to the confocal image. The nucleus is just below, at the limit of the confocal section. The last column of each panel represents Na_v1.5 channels (blue). (b,c) Contractile structural organization characteristics studied using AutoTT software⁵⁴. The cell area covered by the mlc2v contractile protein (b) and organizational integrity (c) were evaluated. (d–g) FACS technique revealed two distinct hiPSC-CM populations comprising mononucleated cells and polynucleated cells (that are described in Figure S7). Histograms summarizing the size (forward scatter, FSC) and granularity (side scatter, SSC) of hiPSC-CMs (WT and R219H) at D20, D30, and D60 of differentiation evaluated using the FACS technique.