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. 2018 Sep 14;8:13819. doi: 10.1038/s41598-018-31952-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transplanted ears develop beyond otocyst stage. (A) Three-dimensional (3D) reconstruction of Hoechst staining of a chicken ear three days after transplant. (B) 3D reconstruction of Hoechst staining of a mouse ear four days after transplant. (C) Lipophilic dye labeling (green) from the alar plate and Hoechst (white) staining of a transplanted chicken ear four days after transplant showing innervation of the ears. The basilar papilla (BP, arrow)/lagena grew perpendicular to the plane of the image and is therefore not visible in this image or in E. (D) Lipophilic dye labeling (green) from the alar plate and Hoechst (white) staining of a transplanted mouse ear four days after transplant showing innervation of the ears. Semicircular canals (Ac, anterior, Pc, posterior, Hc, horizontal) are labeled in the native chicken ear. (C’) Higher magnification of C (boxed area) showing innervation of the posterior canal (Pc) utricle (U) and saccule (S) from the transplanted ear. (D’) Higher magnification of D (boxed area) showing otic ganglia (G) adjacent to the mouse ear. Orientation for all panels is as in A (A, anterior, D, dorsal, M, medial). Scale bars represent 100 μm.