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. 2018 Sep 14;9:3760. doi: 10.1038/s41467-018-05767-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Quantitative lipidome analyses of liver and plasma from GF and SPF mice. Data from experiment 1 are shown (n = 6/6). Candidates verified in experiment 2 (SPF: n = 14; GF: n = 12) are displayed in blue (high in SPF) or orange (high in GF). a Total fatty acids (FA) in liver. b PC species in liver. c Total FA in plasma. d PC species in plasma. a–d Volcano plots: significance and log₂ fold change in individual lipid species; boxplots: concentrations of saturated (SA), monounsaturated (MU), and polyunsaturated (PU) lipid species in GF and SPF mice; barplots: molecular lipid species profile of GF animals. In boxplots the thick lines represent the medians, the upper and lower lines of the boxes show the 25 and 75% quartiles and the whiskers are 1.5 times the interquartile range of the data. Error bars in the bar plots show the standard deviation. e Lipid class composition of liver samples, experiment 1. f Sphingolipid composition of liver samples, experiment 1. g Lipid class composition of plasma, experiment 1. GF: germfree, PC: phosphatidylcholine (diacyl), PC: O phosphatidylcholine (alkyl-acyl), SPF: specific pathogen-free, for other abbreviations, see Table 1