Table 1.
Summary of lipids quantified in plasma and liver samples from GF and SPF animals using mass-spec–based methods
| Lipid category | Analyzed species | Species detected in liver | Species detected in plasma | Lipid class | Method |
|---|---|---|---|---|---|
| Total FA | 33 | 27 | 21 | Total fatty acids (FA) | GC-MS |
| Glycero-phospholipids | 377 | 222 | 133 | Phosphatidylcholine (PC)a Phosphatidylethanolamine (PE)a Phosphatidylserine (PS)a Phosphatidylglycerol (PG)a Phosphatidylinositol (PI)a PE-based plasmalogens (PE P)a Lyso-PC (LPC)a Phosphatidic acid (PA)bc Lyso-PA (LPA)bc Lyso-PG (LPG)bc Cardiolipin (CL)bc Bis(monacylglycero)phosphate (BMP)bc |
aESI-MS-MS bLC-MS/MS |
| Sphingolipids (SL) | 77 | 42 | 33 | Sphingomyelin (SM)a Ceramide (Cer)a Dihexosylceramide (Hex2Cer)bc; Hexosylceramide (HexCer)bc Sphinganine (SPA) Sphingosine (SPH)bc Phytosphingosine (Phyto-SPH) Sphingosine-1-phosphate (S1P)bc Sphingosylphosphorylcholine (SPC)bc |
aESI-MS-MS bLC-MS/MS |
| Sterols | 38 | 23 | 23 | Free Cholesterol (FC) Cholesterylester (CE) |
ESI-MS/MS |
ESI: electrospray ionization, GC: gas chromatography, LC: liquid chromatography, MS/MS: tandem mass spectrometry
cNot analyzed in plasma