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. 2018 Sep 14;8:13815. doi: 10.1038/s41598-018-32164-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the effect of gene disruption on cell growth. The various T. kodakarensis wild type (KU216) and mutant strains were grown in ASW-YT medium. Cultures were carried out at the optimal growth temperature of 85 °C, or at 75 °C and 90 °C, and cell growth was monitored by counting the number of cells with Thoma’s cell counter during the course of the culture. The mean values obtained from three independent experiments and the standard deviations are shown with bars. Significance values of *p < 0.05 relative to KU216. square: KU216, diamond: Δcbf5, triangle: Δpus10, circle: Δnop10, and cross: Δgar1.