Table 2.
Provides an example of comparing two non-culture based detection systems for Salmonella.
Non-culture based microbial detection | NMR Nanotech (microbes, nucleic acids, proteins) | PCR (Salmonella) |
---|---|---|
Detection method | PCR-NMR | Isothermal PCR |
Sample-to-answer time | <1 h | 10–26 h |
Sample type | Food, tissue, water, soil, feces | Fish tissue |
Sample size | 1–325 g (food); 0.1 g (eg. shrimp tissue) | 25–325 g |
Limits of detection (LOD) | 10ˆ3 colony forming unit (CFU)/mL post-enrichment; 1–10 CFU/analytical unit | 10ˆ4 CFU/mL post-enrichment; 1 CFU/analytical unit |
Sensitivity | >98% | 98.7% |
Specificity | Near 100% | 98.3% |