Fig. 2.

Let-7b promotes differentiation of keratinocytes in the let-7b^TG mouse model. a Let-7b expression was detected by real-time PCR in wild-type mice treated with IMQ for 0, 1, 3, 5, 7 days. b Immunofluorescence staining with Ki67 (red) antibody was carried out at day 5 after treated with IMQ in wild-type and transgenic mice. Nuclei were stained with DAPI (blue signal). No noticeable difference was found in percentage of Ki67 positive keratinocytes in wild-type and transgenic mice. Scale Bar = 200 μm. c Immunohistochemistry analysis the expression of CK10 and involucrin in normal subjects (left) and psoriatic patients (right). scale Bar = 100 μm. d Western blot analysis of epidermal differentiation markers (Keratin 10 and Involucrin) in the epidermis of normal skin derived from twelve healthy individuals (N1 to 12) or in psoriatic lesions derived from twelve patients with psoriasis (P1 to 12). The columnar was relative quantitation of CK10 and involucrin protein levels in twelve different samples, which were shown after normalization to the endogenous control of GAPDH (Mean ± SD). **P<0.01. e Real-time PCR analysis of Involucrin and Filaggrin expression in the epidermis of wild-type and transgenic mice treated with IMQ or vehicle. f Western blot analysis of epidermal differentiation markers (Keratin 10 and K1) in wild-type and transgenic mice treated with IMQ for 0, 3, 5 days. The columnar was relative quantitation of CK10 and involucrin protein levels, which were shown after normalization to the endogenous control of GAPDH (Mean ± SD). *P<0.05; **P<0.01.